abstract：BACKGROUND:The hepatocyte-derived acute-phase reactant C-reactive protein (CRP) has been the subject of intense research over the last 2 decades for its possible role in the pathogenesis of cardiovascular diseases. This research has spawned interest in the use of the blood concentration of CRP for predicting a first co...

journal_title：Clinical chemistry

authors： Hingorani AD,Shah T,Casas JP,Humphries SE,Talmud PJ

更新日期：2009-02-01 00:00:00

abstract：BACKGROUND:Cross-sectional studies have reported seasonal variation in high-sensitivity C-reactive protein (hsCRP). However, longitudinal data are lacking. METHODS:We collected data on diet, physical activity, psychosocial factors, physiology, and anthropometric measurements from 534 healthy adults (mean age 48 years,...

journal_title：Clinical chemistry

authors： Chiriboga DE,Ma Y,Li W,Stanek EJ 3rd,Hébert JR,Merriam PA,Rawson ES,Ockene IS

更新日期：2009-02-01 00:00:00

abstract：BACKGROUND:C-reactive protein (CRP), the prototypic marker of inflammation, is present in atherosclerotic plaques and appears to promote atherogenesis. Also, CRP has been localized to monocytes and tissue macrophages, which are present in the necrotic core of lesions prone to plaque rupture. Leukocyte-derived myelopero...

journal_title：Clinical chemistry

authors： Singh U,Devaraj S,Jialal I

更新日期：2009-02-01 00:00:00

abstract：BACKGROUND:Single-nucleotide polymorphisms (SNPs) in inflammation-related genes have been linked to an increased risk of ischemic stroke. Most of these SNP results have not been replicated, however, and metaanalyses of the effects of inflammation-related genes are rare. We investigated 49 SNPs in 34 genes previously re...

journal_title：Clinical chemistry

authors： Greisenegger S,Zehetmayer S,Bauer P,Endler G,Ferrari J,Lang W,Janisiw M,Steiner L,Cheng S,Lalouschek W,Mannhalter C

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Lysosomal storage disorders (LSDs) comprise more than 40 genetic diseases that result in the accumulation of products that would normally be degraded by lysosomal enzymes. A tandem mass spectrometry (MS/MS)-based method is available for newborn screening for 5 LSDs, and many laboratories are initiating pilot...

journal_title：Clinical chemistry

authors： De Jesus VR,Zhang XK,Keutzer J,Bodamer OA,Mühl A,Orsini JJ,Caggana M,Vogt RF,Hannon WH

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:TMPRSS2:ERG fusions are promising prostate cancer biomarkers. Because they can occur in multiple forms in a single cancer specimen, we developed a quantitative PCR test that detects both type III and type VI TMPRSS2:ERG fusions. The assay is quantified from a standard curve determined with a plasmid-cloned t...

journal_title：Clinical chemistry

authors： Clark JP,Munson KW,Gu JW,Lamparska-Kupsik K,Chan KG,Yoshida JS,Kawachi MH,Crocitto LE,Wilson TG,Feng Z,Smith SS

更新日期：2008-12-01 00:00:00

abstract：BACKGROUND:Meconium analysis is a diagnostically sensitive and objective alternative to maternal self-report for detecting prenatal tobacco exposure. Nicotine and metabolite disposition in meconium is poorly characterized, and correlation of analytes' concentrations with neonatal outcomes is unexplored. Our objectives ...

journal_title：Clinical chemistry

authors： Gray TR,Magri R,Shakleya DM,Huestis MA

更新日期：2008-12-01 00:00:00

abstract：BACKGROUND:In the workup of patients with suspected hereditary nonpolyposis colorectal cancer (HNPCC), detection of loss of heterozygosity (LOH) could help pinpoint the mismatch-repair (MMR) gene carrying the germline mutation, but analysis of microsatellite markers has proved unreliable for this purpose. We developed ...

journal_title：Clinical chemistry

authors： Bujalkova M,Zavodna K,Krivulcik T,Ilencikova D,Wolf B,Kovac M,Karner-Hanusch J,Heinimann K,Marra G,Jiricny J,Bartosova Z

更新日期：2008-11-01 00:00:00

abstract：BACKGROUND:Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. METHODS:We developed a method for measuring serum testosterone (T) and 5alpha-dihydrotestosterone (DHT) simultaneously via liquid-liquid extraction followed by liquid chromatography-tandem ma...

journal_title：Clinical chemistry

authors： Shiraishi S,Lee PW,Leung A,Goh VH,Swerdloff RS,Wang C

更新日期：2008-11-01 00:00:00

abstract：BACKGROUND:Reports of the use of multiplex enzyme assay screening for Pompe disease, Fabry disease, Gaucher disease, Niemann-Pick disease types A and B, and Krabbe disease have engendered interest in the use of this assay in newborn screening. We modified the assay for high-throughput use in screening laboratories. ME...

journal_title：Clinical chemistry

authors： Zhang XK,Elbin CS,Chuang WL,Cooper SK,Marashio CA,Beauregard C,Keutzer JM

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:Microalbuminuria (MA) is recognized as an important risk factor for cardiovascular and renal complications in diabetes. We sought to evaluate how screening for MA is conducted and how urine albumin (UA) results are interpreted in primary care internationally. METHODS:General practitioners (GPs) received a c...

journal_title：Clinical chemistry

authors： Aakre KM,Thue G,Subramaniam-Haavik S,Bukve T,Morris H,Müller M,Lovrencic MV,Plum I,Kallion K,Aab A,Kutt M,Gillery P,Schneider N,Horvath AR,Onody R,Oosterhuis W,Ricos C,Perich C,Nordin G,Sandberg S

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:The accurate and precise measurement of urinary albumin is critical, since even minor increases are diagnostically sensitive indicators of renal disease, cardiovascular events, and risk for death. To gain insights into potential measurement biases, we systematically compared urine albumin measurements perfor...

journal_title：Clinical chemistry

authors： Shaikh A,Seegmiller JC,Borland TM,Burns BE,Ladwig PM,Singh RJ,Kumar R,Larson TS,Lieske JC

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:Coffee consumption has been associated with several risk factors for coronary heart disease, including increased cholesterol, increased blood pressure, and increased plasma total homocysteine (tHcy). tHcy is determined by several B-vitamins. However, reports about the association between coffee intake and B-...

journal_title：Clinical chemistry

authors： Ulvik A,Vollset SE,Hoff G,Ueland PM

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:Risk assessments of patients should be based on objective variables, such as biological markers that can be measured routinely. The acute response to stress causes the release of catecholamines from the adrenal medulla accompanied by chromogranin A (CGA). To date, no study has evaluated the prognostic value ...

journal_title：Clinical chemistry

authors： Zhang D,Lavaux T,Voegeli AC,Lavigne T,Castelain V,Meyer N,Sapin R,Aunis D,Metz-Boutigue MH,Schneider F

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:The recent interest of clinical laboratories in developing serum testosterone assays based on isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) stems from the lack of accuracy of direct immunoassays. In this study, we assessed the accuracy and state of standardization (traceabilit...

journal_title：Clinical chemistry

authors： Thienpont LM,Van Uytfanghe K,Blincko S,Ramsay CS,Xie H,Doss RC,Keevil BG,Owen LJ,Rockwood AL,Kushnir MM,Chun KY,Chandler DW,Field HP,Sluss PM

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Improved methods are required to screen drug candidates for their influences on protein interactions. There is also a compelling need for miniaturization of screening assays, with attendant reductions in reagent consumption and assay costs. METHODS:We used sensitive, miniaturized proximity ligation assays (...

journal_title：Clinical chemistry

authors： Gustafsdottir SM,Wennström S,Fredriksson S,Schallmeiner E,Hamilton AD,Sebti SM,Landegren U

更新日期：2008-07-01 00:00:00

abstract：BACKGROUND:direct DNA sequencing is the primary clinical technique for identifying mutations in human disease, but sequencing often does not detect intragenic or whole-gene deletions. Oligonucleotide array-based comparative genomic hybridization (CGH) is currently in clinical use to detect major changes in chromosomal ...

journal_title：Clinical chemistry

authors： Wong LJ,Dimmock D,Geraghty MT,Quan R,Lichter-Konecki U,Wang J,Brundage EK,Scaglia F,Chinault AC

更新日期：2008-07-01 00:00:00

abstract：BACKGROUND:SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS:We used SELDI-TOF MS to analyze serum samples from patients with various forms of inflammatory arthritis. Several protein profiles were collected on d...

journal_title：Clinical chemistry

authors： de Seny D,Fillet M,Ribbens C,Marée R,Meuwis MA,Lutteri L,Chapelle JP,Wehenkel L,Louis E,Merville MP,Malaise M

更新日期：2008-06-01 00:00:00

abstract：BACKGROUND:C-peptide is a marker of insulin secretion in diabetic patients. We assessed within- and between-laboratory imprecision of C-peptide assays and determined whether serum calibrators with values assigned by mass spectrometry could be used to harmonize C-peptide results. METHODS:We sent 40 different serum samp...

journal_title：Clinical chemistry

authors： Little RR,Rohlfing CL,Tennill AL,Madsen RW,Polonsky KS,Myers GL,Greenbaum CJ,Palmer JP,Rogatsky E,Stein DT

更新日期：2008-06-01 00:00:00

abstract：BACKGROUND:Bilirubin has antioxidative and cytoprotective properties. Low plasma concentrations of bilirubin are reportedly associated with the development of coronary and cerebrovascular disease, and bilirubin concentrations are strongly correlated with the enzyme activity of the hepatic uridine diphosphate glucuronos...

journal_title：Clinical chemistry

authors： Rantner B,Kollerits B,Anderwald-Stadler M,Klein-Weigel P,Gruber I,Gehringer A,Haak M,Schnapka-Köpf M,Fraedrich G,Kronenberg F

更新日期：2008-05-01 00:00:00

abstract：BACKGROUND:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone that exists in multiple forms. Immunoassays commonly used in clinical laboratories measure intact hCG, total beta hCG (intact hCG + hCG free beta-subunit), and/or hCG free beta-subunit. Measurement of serum concentrations of hCG is us...

journal_title：Clinical chemistry

authors： Cao ZT,Rej R

更新日期：2008-04-01 00:00:00

abstract：BACKGROUND:Optimal cutoff values for tests results involving continuous variables are often derived in a data-driven way. This approach, however, may lead to overly optimistic measures of diagnostic accuracy. We evaluated the magnitude of the bias in sensitivity and specificity associated with data-driven selection of ...

journal_title：Clinical chemistry

authors： Leeflang MM,Moons KG,Reitsma JB,Zwinderman AH

更新日期：2008-04-01 00:00:00

abstract：BACKGROUND:Paraoxonase may mitigate oxidative damage and thus lower risk of macrovascular disease. METHODS:DNA samples from 2252 participants in the Coronary Artery Risk Development in Young Adults (CARDIA) study were genotyped for the L55M and Q192R polymorphisms of the PON1 (paraoxonase 1) gene, and paraoxonase acti...

journal_title：Clinical chemistry

authors： Thyagarajan B,Jacobs DR Jr,Carr JJ,Alozie O,Steffes MW,Kailash P,Hayes JH,Gross MD

更新日期：2008-04-01 00:00:00

abstract：BACKGROUND:Insulin, growth hormone (GH), and growth factors (insulin-like growth factors [IGFs] and their binding proteins [IGFBPs]) are known to influence fetal growth and also the synthesis/secretion of the recently discovered hormones leptin and ghrelin. METHODS:In 153 delivering mothers and their offspring at birt...

journal_title：Clinical chemistry

authors： Chiesa C,Osborn JF,Haass C,Natale F,Spinelli M,Scapillati E,Spinelli A,Pacifico L

更新日期：2008-03-01 00:00:00

abstract：BACKGROUND:Little is known about lipoprotein(a) [Lp(a)] as a predictor of vascular events among ethnic Chinese. We prospectively investigated the association of Lp(a) with cardiovascular disease and all-cause death in a community-based cohort. METHODS:We conducted a community-based prospective cohort study of 3484 par...

journal_title：Clinical chemistry

authors： Chien KL,Hsu HC,Su TC,Sung FC,Chen MF,Lee YT

更新日期：2008-02-01 00:00:00

abstract：BACKGROUND:The analysis of bodily fluids using SELDI-TOF MS has been reported to identify signatures of spectral peaks that can be used to differentiate patients with a specific disease from normal or control patients. This report is the 2nd of 2 companion articles describing a validation study of a SELDI-TOF MS approa...

journal_title：Clinical chemistry

authors： McLerran D,Grizzle WE,Feng Z,Thompson IM,Bigbee WL,Cazares LH,Chan DW,Dahlgren J,Diaz J,Kagan J,Lin DW,Malik G,Oelschlager D,Partin A,Randolph TW,Sokoll L,Srivastava S,Srivastava S,Thornquist M,Troyer D,Wright GL

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The association of changes in oxidative and proinflammatory states with vascular function after diet and exercise intervention among obese children has not been previously explored. METHODS:In this 6-week diet and exercise intervention study in 35 obese children, age 12 to 18 years, we evaluated the relatio...

journal_title：Clinical chemistry

authors： Kelishadi R,Hashemi M,Mohammadifard N,Asgary S,Khavarian N

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting ana...

journal_title：Clinical chemistry

authors： Seipp MT,Pattison D,Durtschi JD,Jama M,Voelkerding KV,Wittwer CT

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation dif...

journal_title：Clinical chemistry

authors： Satterfield BC,Kulesh DA,Norwood DA,Wasieloski LP Jr,Caplan MR,West JA

更新日期：2007-12-01 00:00:00

abstract：BACKGROUND:New genetic forms of hereditary hemochromatosis (HH) or hereditary hyperferritinemia (HF) have been identified over the last few years, and abnormalities of various genes may interact in a single patient. This study aimed to develop a rapid automated method for sequencing the main genes involved. METHODS:We...

journal_title：Clinical chemistry

authors： Cunat S,Giansily-Blaizot M,Bismuth M,Blanc F,Dereure O,Larrey D,Quellec AL,Pouderoux P,Rose C,Raingeard I,Renard E,Schved JF,Aguilar-Martinez P,CHU Montpellier AOI 2004 Working Group.

更新日期：2007-12-01 00:00:00

abstract：BACKGROUND:Buprenorphine is under investigation as a pharmacotherapeutic agent for treating opioid dependence in pregnant women. We hypothesized that there would be a relationship between the cumulative maternal dose of buprenorphine during pregnancy and the concentration of buprenorphine and norbuprenorphine in matern...

journal_title：Clinical chemistry

authors： Goodwin RS,Wilkins DG,Averin O,Choo RE,Schroeder JR,Jasinski DR,Johnson RE,Jones HE,Huestis MA

更新日期：2007-12-01 00:00:00

abstract：BACKGROUND:The discovery of cell-free fetal DNA in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methylation-sensitive restriction endonucleases are commonly u...

journal_title：Clinical chemistry

authors： Tong YK,Chiu RW,Leung TY,Ding C,Lau TK,Leung TN,Lo YM

更新日期：2007-11-01 00:00:00

abstract：BACKGROUND:Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for sing...

journal_title：Clinical chemistry

authors： Soni GV,Meller A

更新日期：2007-11-01 00:00:00

abstract：BACKGROUND:Gas6 is a vitamin K-dependent antiapoptotic protein that has been implicated in cardiovascular pathophysiology. We report the development and validation of an ELISA for Gas6, and the variation of plasma Gas6 with hormonal status in a study designed to evaluate the effect of oral contraception on plasma marke...

journal_title：Clinical chemistry

authors： Clauser S,Peyrard S,Gaussem P,Crespin M,Emmerich J,Aiach M,Borgel D

更新日期：2007-10-01 00:00:00

abstract：BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation ac...

journal_title：Clinical chemistry

authors： Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret P

更新日期：2007-10-01 00:00:00

abstract：BACKGROUND:Protein tyrosine phosphatase 1B (PTPN1) dephosphorylates insulin receptors and attenuates insulin signaling. Polymorphisms in the coding sequence of PTPN1 have been variably associated with type 2 diabetes (T2D). We hypothesized that variations within the PTPN1 promoter might contribute to the development of...

journal_title：Clinical chemistry

authors： Meshkani R,Taghikhani M,Al-Kateb H,Larijani B,Khatami S,Sidiropoulos GK,Hegele RA,Adeli K

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:Increased concentrations of cell-free DNA have been found in several disorders and have been interpreted as evidence of increased rates of cell death or turnover. Evidence from in vitro and animal experiments suggests that DNA may play a role in the pathogenesis of rheumatoid arthritis (RA). METHODS:We meas...

journal_title：Clinical chemistry

authors： Zhong XY,von Mühlenen I,Li Y,Kang A,Gupta AK,Tyndall A,Holzgreve W,Hahn S,Hasler P

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. METHODS:We compared 4 common DNA extraction ...

journal_title：Clinical chemistry

authors： Sjöholm MI,Dillner J,Carlson J

更新日期：2007-08-01 00:00:00

abstract：BACKGROUND:A genomic deletion of exon 3 (d3-GHR) of the growth hormone (GH) receptor (GHR) has been linked to the effectiveness of GH therapy in children with GH deficiency. Carriers of the d3-GHR genotype had higher GH-induced growth rates than children homozygous for the full-length (fl)-GHR. The aim of this study wa...

journal_title：Clinical chemistry

authors： Schmid C,Krayenbuehl PA,Bernays RL,Zwimpfer C,Maly FE,Wiesli P

更新日期：2007-08-01 00:00:00

abstract：BACKGROUND:Plant-derived cardenolides reportedly possess anticancer properties in human leukemic cells via selective induction of apoptosis, cell cycle arrest, and differentiation. Selective induction of apoptosis with mammalian-derived digoxin-like immunoreactive factor (DLIF) could provide new strategies for anticanc...

journal_title：Clinical chemistry

authors： Ihenetu K,Qazzaz HM,Crespo F,Fernandez-Botran R,Valdes R Jr

更新日期：2007-07-01 00:00:00